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How to order

Use the following forms and product guide to avail the respective services.
  • Oligonucleotide Synthesis
    Samples are processed on a first come, first serve basis. We are generally able to provide results within 1 to 2 weeks. Please inquire about expected turnaround times when submitting samples. Please use the following forms for the purpose.
    --Service Request Form for Oligonucleotide synthesis Spreadsheet
  • Custom Sequencing
    Samples are processed on a first come, first serve basis. We are generally able to provide results within 5-7working days. Please inquire about expected turnaround times when submitting samples. Please use the following forms for the purpose.
    --Service Request Form for Sequencing Spreadsheet
  • Fragment Analysis
    If you want to submit genomic DNA or RNA, please ensure the extracted template is of sufficient quantity and of quality good enough for PCR or RT-PCR. If you plan to use whole blood or blood component for template extraction, please collect blood in BD lavender top vacutainers or ensure that blood is collected in EDTA. Do not use Heparin for blood collection. For nay other tissue, please ensure the samples are of sufficient quality and quantity for DNA or RNA extraction suitable for PCR. Please consult Technical Support for any further details with your specific query
    --Service Request Form for Fragment analysis
  • Genotyping by Massarray
    Samples are processed on a first come, first serve basis.Please inquire about expected turnaround times when submitting samples. Please use the Sample Submission Form available on this website.
    --Service Request Form for Massarray SNP Genotyping Spreadsheet
  • Proteomics
    Samples are processed on a first come, first serve basis. We are generally able to provide results within 2 to 3 weeks. Please inquire about expected turnaround times when submitting samples. Please use the following forms for the purpose.
    --Service Request Form for 2 D electrophoresis and Mass Spectrometry Spreadsheet

Directory of Service

Sl. No. Service Code Description Units Turn Around Time Sample Requirement
Oligonucleotide Synthesis Facility
1 TCGA-O001

Desalted oligo 50 nmol synthesis scale, OD 4 to 12, QC by MALDI-ToF (17-32 mer)

Base 5 working Days  
2 TCGA-O002

Degenerate oligo 50 nmol synthesis scale,QC by MALDI-ToF(17-32 mer)

Base 7 working Days  
3 TCGA-O003

PAGE purified oligo 50 nmol synthesis scale, OD 2 to 8, QC by MALDI-ToF (17-32 mer)

Base+Oligo 10 Working Days  
4 TCGA-O004

Desalted oligo 200 nmol synthesis scale, OD 8 to 20,QC by MALDI-ToF (17-32 mer)

Base 5 Working Days  
5 TCGA-O005

Degenerate oligo 200 nmol synthesis scale, QC by MALDI-ToF(17-32 mer)

Base    
6 TCGA-O006

PAGE purified oligo 200 nmol synthesis scale, OD 4 to 12, QC by MALDI-ToF (17-32 mer)

Base+Oligo 10 Working Days  
7 TCGA-O007

5' Fluorescent labeled oligo ( HEX,6-FAM, or TET labeled), QC by MALDI-ToF (17-32 mer)

Base+Oligo 15 Working Days  

Proteomics Facility

1 TCGA-P001

2-D gel electrophoresis of protein samples

Sample 3-4 weeks/depends upon project nature 500ug to 1mg(to be discussed prior to sending the sample)
2 TCGA-P002

Peptide mass fingerprinting (PMF) by MALDI-TOF of Tryptic digests of protein

Sample 10Working Days Tryptic digest in lyophillized/liquid form
3 TCGA-P003

Peptide mass fingerprinting and MS /MS of 2-3 peptides from PMF by MALDI-TOF/TOF

Sample 10Working Days Tryptic digest in lyophillized/liquid form
4 TCGA-P004

MS/MS of Tryptic digests of proteins using nano LC-MS

Sample 10Working Days Tryptic digest in lyophillized/liquid form
5 TCGA-P005

Alkylation, Reduction ,Tryptic Digestion & peptide mass fingerprinting by MALDI-TOF

Sample 10-15 Working Days Coomassie /silver stained band (preferably coomassie)
6 TCGA-P006

Alkylation ,Reduction,Tryptic Digestion & MS /MS of 2-3 peptides from PMF for by MALDI-TOF/TOF

Sample 10-15 Working Days Coomassie /silver stained band (preferably coomassie)
7 TCGA-P007

Alkylation ,Reduction,Tryptic Digestion and MS /MS using nano LC-MS

Sample 10-15 Working Days Coomassie /silver stained band
8 TCGA-P008

Molecular Weight of Intact Protein

Sample 5 Working Days lyophillized powder, Protein (5-60KD)- 30-50 pmol,Protein (60KD and above)- 80-100pmol
9 TCGA-P009

Molecular Weight of Peptide

Sample 5 Working Days lyophillized powde , Peptides- 5pmol/ µl (above 700Da)
10 TCGA-P010

1-D-Gel Electrophoresis

Sample 7 Working Days 100ug
11 TCGA-P011

Insolution digestion, Peptide mass fingerprinting and MS/MS by MALDI-TOF/TOF

Sample 10-15 Working Days 10-20ug
12 TCGA-P012

Insolution digestion and MS/MS of Tryptic digests using nano LC-MS

Sample 10-15 Working Days 10-20ug
13 TCGA-P013

Sample preparation for 2-D gel electrophoresis of Bacterial samples

Sample To be done prior to 2D gel electrophoresis(TCGA-P001) lyophillized powder
14 TCGA-P014

Sample preparation for 2-D gel electrophoresis of Plant samples

Sample To be done prior to 2D gel electrophoresis(TCGA-P001) lyophillized powder
15 TCGA-P015

Sample preparation for 2-D gel electrophoresis of Plasma samples

Sample To be done prior to 2D gel electrophoresis(TCGA-P001) lyophillized powder
16 TCGA-P016

Sample preparation for 2-D gel electrophoresis of Tissue samples

Sample To be done prior to 2D gel electrophoresis(TCGA-P001) lyophillized powder
17 TCGA-P017

Zip Tip Clean-up

Tip To be done prior to MALDI from service no. (TCGA-P002/P003/ P005/P006)
18 TCGA-P018

Analysis of 2-D gels using PD Quest

Sample To be done after 2 D gel electrophoresis(TCGA-P001) Gel pictures
19 TCGA-P019

MUD PIT Analysis using 2D nano LC-MS

Sample    
20 TCGA-P020

MS /MS of additional peptides from PMF data using MALDI-TOF/TOF ( per peptide) for sequencing

Sample To be done along with TCGA- P003/P006 from service no. (TCGA-P003/ P006)
21 TCGA-P021

Iso electric focusing

Sample 10 Working Days 100-200ug
22 TCGA-P022

Transfer of protein from 1D/2D Gel to PVDF membrane

Sample 7-10 Working Days  

Genotyping Facility

1 TCGA-G001 Mass Array Sequenom High Throughput Genotyping Genotype Depends on project Depends on project
2 TCGA-G002 Assay Design and Optimization Locus Depends on project Not Required
3 TCGA-G003 Assay Design, Optimization and Validation (48 samples) Locus Depends on project Not Required
4 TCGA-G004 Picogreen DNA quantification Sample 5 Working Days Approximately 50 ng Genomic DNA
5 TCGA-G005 Affymetrix Genechip Genotyping (End to End) Sample Depends on project Depends on project(500-1000ng genomic DNA)
6 TCGA-G006 Affymetrix Genechip Genotyping Hybridization and Scanning Service Sample Depends on project Depends on project(500-1000ng genomic DNA)
7 TCGA-G007 Illumina Goldengate Genotyping (384, 768 and 1536 Plex) Genotype Depends on project 500 ng Genomic DNA
8 TCGA-G008 Illumina Whole Genome Infinium Assays Sample Depends on project Depends on project(500-1000ng genomic DNA)

Microarray Facility 

1 TCGA-M001 RNA isolation (Blood , Tissue  culture cells & lymphocytes) Blood or tissue or culture cells or lymphocytes ** This service is only available with TCGA-M004 , TCGA-M005, TCGA-M006, TCGA-M007, TCGA-M008 and TCGA-M009
2 TCGA-M002 RNA Quality check on Agilent Bioanalyser 2100 RNA sample 2 Working Days Depends upon the Assay
3 TCGA-M003 DNA Quality Check on Agilent Bioanalyser 2100 DNA sample 2 Working Days Depends upon the Assay
4 TCGA-M004 Affymetrix GeneChip Eukaryotic Expression Standard Protocol RNA sample Depends upon project Total RNA atleast 6ug in 10 ul
5 TCGA-M005 Affymetrix GeneChip Eukaryotic Expression small sample protocol RNA sample Depends upon project Total RNA atleast 150ng in 10 ul
6 TCGA-M006 Illumina BeadChip Expression protocol RNA sample Depends upon project Total RNA atleast 500ng in 11 ul
7 TCGA-M007 Illumina BeadChip miRNA Gene Expression RNA sample Depends upon project Total RNA atleast 200ng in 11ul
8 TCGA-M008 mRNA quantification by Real time PCR using SYBR Green RNA sample Depends upon project Consultation recommended prior to assay design
9 TCGA-M009 mRNA quantification by Real time PCR using probes RNA sample Depends upon project Consultation recommended prior to assay design
10 TCGA-M010 Premium Analysis Service (PAS) Data Depends upon project Consultation essential prior to analysis
11 TCGA-M011 Full Analysis Service (FAS) Data Depends upon project Consultation essential prior to analysis

Sequencing Facility

1 TCGA-S001 Custom DNA Sequencing (Single/Double pass) Reaction 5 Working Days 1ug Plasmid,500ng PCR Product
2 TCGA-S002 Consolidated DNA Sequencing Reaction Depends upon project Genomic DNA
3 TCGA-S003 High Throughput DNA Sequencing Reaction 5 working Days 96 or more DNA samples in plates
4 TCGA-S004 24Hour DNA Sequencing Reaction 24 hours (1 working day) 1ug Plasmid,500ng PCR Product
5 TCGA-S005 Primer Walking Kb 15 working Days 1ug DNA
6 TCGA-S006 Microbial Identification-(using 16S, 18S & 28S rDNA) Sample 7 working Days 5 ml culture
7 TCGA-S007 TA cloning Sample Depends upon project Genomic DNA
8 TCGA-S008 Custom cloning Sample Depends upon project Genomic DNA
9 TCGA-S009 Custom Fragment Analysis Sample 7 working Days 200ng genomic DNA
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