TCGA -- How To Order

How to order

Use the following forms and product guide to avail the respective services.

Oligonucleotide Synthesis
Samples are processed on a first come, first serve basis. We are generally able to provide results within 1 to 2 weeks. Please inquire about expected turnaround times when submitting samples. Please use the following forms for the purpose.
--Service Request Form for Oligonucleotide synthesis Spreadsheet

Custom Sequencing
Samples are processed on a first come, first serve basis. We are generally able to provide results within 5-7working days. Please inquire about expected turnaround times when submitting samples. Please use the following forms for the purpose.
--Service Request Form for Sequencing Spreadsheet

Fragment Analysis
If you want to submit genomic DNA or RNA, please ensure the extracted template is of sufficient quantity and of quality good enough for PCR or RT-PCR. If you plan to use whole blood or blood component for template extraction, please collect blood in BD lavender top vacutainers or ensure that blood is collected in EDTA. Do not use Heparin for blood collection. For nay other tissue, please ensure the samples are of sufficient quality and quantity for DNA or RNA extraction suitable for PCR. Please consult Technical Support for any further details with your specific query
--Service Request Form for Fragment analysis

Genotyping by Massarray
Samples are processed on a first come, first serve basis.Please inquire about expected turnaround times when submitting samples. Please use the Sample Submission Form available on this website.
--Service Request Form for Massarray SNP Genotyping Spreadsheet

Microarray Facility
--Data analysis and report generation form Spreadsheet

Proteomics
Samples are processed on a first come, first serve basis. We are generally able to provide results within 2 to 3 weeks. Please inquire about expected turnaround times when submitting samples. Please use the following forms for the purpose.
--Service Request Form for 2 D electrophoresis and Mass Spectrometry Spreadsheet

Directory of Service

Sl. No.	Service Code	Description	Units	Turn Around Time	Sample Requirement
Oligonucleotide Synthesis Facility
1	TCGA-O001	Desalted oligo 50 nm Scale,OD 4 to 12 (>20 mer),QC by MALDI-ToF	Base	5 working Days
2	TCGA-O002	Degenrate oligo 50 nm Scale	Oligo	7 working Days
3	TCGA-O003	5' Fluorescent labelled oligo ( HEX,6-FAM, or TET labelled)	Oligo	15 Working Days
Proteomics Facility
1	TCGA-P001	2-D gel electrophoresis of protein samples	Sample	3-4 weeks/depends upon project nature	500ug to 1mg(to be discussed prior to sending the sample)
2	TCGA-P002	Peptide mass fingerprinting (PMF) by MALDI-TOF of Tryptic digests of protein	Sample	10Working Days	Tryptic digest in lyophillized/liquid form
3	TCGA-P003	Peptide mass fingerprinting and MS /MS of 2-3 peptides from PMF by MALDI-TOF/TOF	Sample	10Working Days	Tryptic digest in lyophillized/liquid form
4	TCGA-P004	MS/MS of Tryptic digests of proteins using nano LC-MS	Sample	10Working Days	Tryptic digest in lyophillized/liquid form
5	TCGA-P005	Alkylation, Reduction ,Tryptic Digestion & peptide mass fingerprinting by MALDI-TOF	Sample	10-15 Working Days	Coomassie /silver stained band (preferably coomassie)
6	TCGA-P006	Alkylation ,Reduction,Tryptic Digestion & MS /MS of 2-3 peptides from PMF for by MALDI-TOF/TOF	Sample	10-15 Working Days	Coomassie /silver stained band (preferably coomassie)
7	TCGA-P007	Alkylation ,Reduction,Tryptic Digestion and MS /MS using nano LC-MS	Sample	10-15 Working Days	Coomassie /silver stained band
8	TCGA-P008	Molecular Weight of Intact Protein	Sample	5 Working Days	lyophillized powder, Protein (5-60KD)- 30-50 pmol,Protein (60KD and above)- 80-100pmol
9	TCGA-P009	Molecular Weight of Peptide	Sample	5 Working Days	lyophillized powde , Peptides- 5pmol/ µl (above 700Da)
10	TCGA-P010	1-D-Gel Electrophoresis	Sample	7 Working Days	100ug
11	TCGA-P011	Insolution digestion, Peptide mass fingerprinting and MS/MS by MALDI-TOF/TOF	Sample	10-15 Working Days	10-20ug
12	TCGA-P012	Insolution digestion and MS/MS of Tryptic digests using nano LC-MS	Sample	10-15 Working Days	10-20ug
13	TCGA-P013	Sample preparation for 2-D gel electrophoresis of Bacterial samples	Sample	To be done prior to 2D gel electrophoresis(TCGA-P001)	lyophillized powder
14	TCGA-P014	Sample preparation for 2-D gel electrophoresis of Plant samples	Sample	To be done prior to 2D gel electrophoresis(TCGA-P001)	lyophillized powder
15	TCGA-P015	Sample preparation for 2-D gel electrophoresis of Plasma samples	Sample	To be done prior to 2D gel electrophoresis(TCGA-P001)	lyophillized powder
16	TCGA-P016	Sample preparation for 2-D gel electrophoresis of Tissue samples	Sample	To be done prior to 2D gel electrophoresis(TCGA-P001)	lyophillized powder
17	TCGA-P017	Zip Tip Clean-up	Tip	To be done prior to MALDI	from service no. (TCGA-P002/P003/ P005/P006)
18	TCGA-P018	Analysis of 2-D gels using PD Quest	Sample	To be done after 2 D gel electrophoresis(TCGA-P001)	Gel pictures
19	TCGA-P019	MUD PIT Analysis using 2D nano LC-MS	Sample
20	TCGA-P020	MS /MS of additional peptides from PMF data using MALDI-TOF/TOF ( per peptide) for sequencing	Sample	To be done along with TCGA- P003/P006	from service no. (TCGA-P003/ P006)
21	TCGA-P021	Iso electric focusing	Sample	10 Working Days	100-200ug
Genotyping Facility
1	TCGA-G001	Mass Array (Sequenom)	Genotype	Depends upon project
2	TCGA-G002	Assay Design and Optimization (Sequenom)	Locus	Depends upon project
3	TCGA-G003	Assay Design, Optimization and Validation (Sequenom)	Locus	Depends upon project
4	TCGA-G004	Genechip Genotyping (Affymetrix)	Samples	Depends upon project	500ng
Microarray Facility
1	TCGA-M001	RNA Assay by Agilent Bioanalyser 2100	Samples	2 Working Days	Depends upon the Assay
2	TCGA-M002	DNA Assay by Agilent Bioanalyser 2100	Samples	2 Working Days	Depends upon the Assay
3	TCGA-M003	GeneChip Eukaryotic Expression Standard Protocol	Samples	Depends upon project	Total RNA atleast 6ug in 10 ul
4	TCGA-M004	GeneChip Eukaryotic Expression small sample protocol	Samples	Depends upon project	Total RNA atleast 150ng in 10 ul
Sequencing Facility
1	TCGA-S001	Custom DNA Sequencing	Reaction	5 Working Days	1ug Plasmid,500ng PCR Product
2	TCGA-S002	PCR Purification	Template	2 working Days
3	TCGA-S003	Plasmid DNA Preparation	Template	2 working Days
4	TCGA-S004	Custom PCR	Template	2 working Days	5 ml culture
5	TCGA-S005	Microbial Identification-(using 16s & 18s rDNA)	Sample	7 working Days	5 ml culture
6	TCGA-S006	Primer Walking	per KB	15 working Days	1ug DNA
7	TCGA-S007	Custom Fragment Analysis	Sample	7 working Days	200ng genomic DNA
8	TCGA-S008	Snapshot	Reaction	6 Working Days	200ng genomic DNA
9	TCGA-S009	24Hour DNA Sequencing	Reaction	24 hours (1 working day)	1ug Plasmid,500ng PCR Product